Generator

Part:BBa_K1312000:Experience

Designed by: Tatsui yusuke   Group: iGEM14_Kyoto   (2014-09-30)

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Please enter how you used this part and how it worked out.

Applications of BBa_K1312000

Intoroduding this part to E. coli cause magnetosome-like vesicle formation in the cells.

User Reviews

mamL, mamQ and mamB are considered to be essential for magnetosome vesicle formation (Fig. 1).

Fig. 1

When we introdued this part to E. coli, the transformants made magnetosome-like vesicles(Fig. 2).

Fig. 2

Transformants only introduced mamL and mamQ also made magnetosome like vesicles. We detected significant difference between negative control and the two transformant.(Fig. 3)

Fig. 3

Additionally, the size of the transformants were larger than control. (Fig. 4)

Fig. 4

Moreover, E. coli growth is retarded when pLQ or pLQB are introduced but no effect on growth rate is detected pL transformant.(Fig. 5)

Fig. 5



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