Deleted
Sample Discontinued
Experience: None
Not Used
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Regulatory

Part:BBa_K131001:Design

Designed by: Kevin McLeod   Group: iGEM08_Calgary_Wetware   (2008-07-22)


ColE2 SOS Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Lab strains of E. coli, such as Top10 from Invitrogen, are deficient in RecA so we required a RecA positive strain to induce this promoter. We used BL21 from Novagen.


Source

This part comes from the ColE2 operon, which came from a plasmid.

References