Part:BBa_K1298005:Design
PgeChia 1-2 (insert) using J04500
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 812
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 722
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 793
Illegal BsaI site found at 1044
Design Notes
The protein code for the PgeChia 1-2 was optimized to remove illegal cut sites. It should be noted that there is no secretory tag in the biobrick, meaning that the chitinase protein is not being transported outside of the cell, but staying inside of it.
Source
BBa_K1298001 (PgeChia 1-1)
- Phytochemistry paper of Dr. Kolosova (1)
- NCBI (protein code) (2)
- Bio Basic Inc. (DNA optimization)
References
1. Kolosova, N. “Cloning and characterization of chitinases from interior spruce and lodgepole pine.” 1 Oct. 2013. Feb. 2014 <http://www.ncbi.nlm.nih.gov/pubmed/24564978>
2. Kolosova, N. "Picea engelmannii x Picea glauca class I chitinase (Chia1-2) mRNA, complete cds." 30 Dec. 2013. Feb. 2014 <http://www.ncbi.nlm.nih.gov/nuccore/HM219844>*
*The sequence found at this link has been optimized to remove illegal cut sites (EcoRI, XbaI, SpeI, PstI) and therefore is not the same as the sequence found on this part page.