Composite

Part:BBa_K1291071:Design

Designed by: İBRAHİM YASİR ORHAN   Group: iGEM14_AUC_TURKEY   (2014-06-23)


HORSERADISH PEROXIDASE TYPE C COMPOSITE PART


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 445
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 565
    Illegal AgeI site found at 719
    Illegal AgeI site found at 1022
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All EcoRI, PstI, SpeI, XbaI restriction sites were removed manually and BamH1 restriction cites were placed at the start and at the end of the composite part.


Source

Horseradish peroxidase Type C region was taken from the horseradish and the other compositions were synthetically merged with this sequence.

References