Composite

Part:BBa_K1290003:Design

Designed by: Dilara Soylu   Group: iGEM14_NGSS_TR   (2014-06-20)


Prom+RBS+OmpA+SpeB Cleavage Site+xylE+double terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1635
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 29
    Illegal NheI site found at 52
    Illegal SpeI site found at 1636
    Illegal PstI site found at 1650
    Illegal NotI site found at 7
    Illegal NotI site found at 1643
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1636
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1636
    Illegal PstI site found at 1650
    Illegal AgeI site found at 588
    Illegal AgeI site found at 960
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

full part


Source

S. Pyogenes, parts registry

References