Plasmid

Part:BBa_K128007:Design

Designed by: Derek Ju   Group: iGEM08_MIT   (2008-10-29)


pLEM415 plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 701
    Illegal XbaI site found at 4102
    Illegal SpeI site found at 4096
    Illegal PstI site found at 707
    Illegal PstI site found at 4078
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 701
    Illegal NheI site found at 2678
    Illegal SpeI site found at 4096
    Illegal PstI site found at 707
    Illegal PstI site found at 4078
    Illegal NotI site found at 4108
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 701
    Illegal BglII site found at 2076
    Illegal BamHI site found at 4090
    Illegal XhoI site found at 668
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 701
    Illegal XbaI site found at 4102
    Illegal SpeI site found at 4096
    Illegal PstI site found at 707
    Illegal PstI site found at 4078
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 701
    Illegal XbaI site found at 4102
    Illegal SpeI site found at 4096
    Illegal PstI site found at 707
    Illegal PstI site found at 4078
    Illegal NgoMIV site found at 129
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5484
    Illegal SapI site found at 4401


Design Notes

Compatible with only a few strains of Lactobacillus. Keep this in mind when attempting to use pLEM415, since Lactobacillus can be picky with its range of plasmids


Source

Donated by Pascale Serror, IRNA in France

References