Plasmid

Part:BBa_K1256014:Design

Designed by: Pei-Hong Chen   Group: iGEM14_Mingdao   (2014-06-10)

pSB1C3-Pred-ccdB-light sensor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 6415
    Illegal suffix found in sequence at 2092
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6415
    Illegal NheI site found at 1619
    Illegal SpeI site found at 2093
    Illegal PstI site found at 2107
    Illegal NotI site found at 2100
    Illegal NotI site found at 6421
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6415
    Illegal BglII site found at 1282
    Illegal BglII site found at 3244
    Illegal BamHI site found at 1949
    Illegal XhoI site found at 3124
    Illegal XhoI site found at 5137
    Illegal XhoI site found at 6291
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 6415
    Illegal suffix found in sequence at 2093
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 6415
    Illegal XbaI site found at 6430
    Illegal SpeI site found at 2093
    Illegal PstI site found at 2107
    Illegal NgoMIV site found at 1296
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1858


Design Notes

Source

References