Plasmid

Part:BBa_K1256012:Design

Designed by: Pei-Hong Chen   Group: iGEM14_Mingdao   (2014-06-10)

pSB1C3-Plac-RFP-Cm-LacI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4447
    Illegal suffix found in sequence at 1078
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4447
    Illegal NheI site found at 223
    Illegal SpeI site found at 1079
    Illegal PstI site found at 1093
    Illegal NotI site found at 1086
    Illegal NotI site found at 4453
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4447
    Illegal BglII site found at 2368
    Illegal BamHI site found at 935
    Illegal XhoI site found at 2110
    Illegal XhoI site found at 4323
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4447
    Illegal suffix found in sequence at 1079
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4447
    Illegal XbaI site found at 4462
    Illegal SpeI site found at 1079
    Illegal PstI site found at 1093
    Illegal AgeI site found at 783
    Illegal AgeI site found at 895
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References