Plasmid_Backbone

Part:BBa_K1256003:Design

Designed by: Pei-Hong Chen   Group: iGEM14_Mingdao   (2014-06-09)

pSB1C3-Plac-SS-NB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found at 1145
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3193
    Illegal NheI site found at 290
    Illegal SpeI site found at 1146
    Illegal PstI site found at 1160
    Illegal NotI site found at 1153
    Illegal NotI site found at 3199
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3193
    Illegal BamHI site found at 1002
    Illegal XhoI site found at 2177
    Illegal XhoI site found at 3069
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3193
    Illegal suffix found at 1146
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3193
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3208
    Illegal SpeI site found at 1146
    Illegal PstI site found at 1160
    Illegal AgeI site found at 850
    Illegal AgeI site found at 962
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

Source

References