Other
Part:BBa_K1253801:Design
Designed by: Rohan Sinha Group: iGEM14_TAS_Taipei (2014-06-27)
Survivin Promoter + GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 944
Design Notes
We designed pSurvivin so that future teams could use it as a promoter that is activated in cancerous cells.
Source
The pSurvivin promoter was cloned from HeLa cells. GFP came from the E0840 part from the 2013 Spring Distribution Kit.