Coding

Part:BBa_K1253500:Design

Designed by: Edward Hsieh   Group: iGEM14_TAS_Taipei   (2014-06-27)


c-Myc coding sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 475
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 475
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 475
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 475
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 307


Design Notes

There is a Pst1 site within the c-Myc coding sequence, so after cloning from the HeLa cell, the PCR sticky end generation(Zeng,1998) method was used to manufacture an EcoR1 site at the beginning, and a Pst1 site at the end of the open reading frame. The reason these sites were generated is in order to place the c-Myc open reading frame on a Biobrick.


Source

This part was cloned from a HeLa cell line.

References