Deleted
Sample Discontinued
Experience: None
Not Used
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Plasmid

Part:BBa_K125010:Design

Designed by: Margaret Ruzicka   Group: iGEM08_Hawaii   (2008-10-26)


Broad-Host-Range RSF1010 derived plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5885
    Illegal suffix found in sequence at 4559
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5885
    Illegal NheI site found at 4735
    Illegal NheI site found at 5684
    Illegal SpeI site found at 4560
    Illegal PstI site found at 4574
    Illegal NotI site found at 4567
    Illegal NotI site found at 5891
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5885
    Illegal BglII site found at 6341
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5885
    Illegal suffix found in sequence at 4560
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5885
    Illegal XbaI site found at 5900
    Illegal SpeI site found at 4560
    Illegal PstI site found at 4574
    Illegal NgoMIV site found at 4299
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6229


Design Notes

PCR amplification was used to isolate the parts from pRL1383a. The oriT,lac promoter, and rbs were all synthesized.


Source

This part is derived from pRL1383a, an RSF1010 derived plasmid and the BioBrick Base Vector.

References