DNA

Part:BBa_K124003:Design

Designed by: Neil J. Parikh   Group: iGEM08_Brown   (2008-07-09)

Bacteriophage 21 Lysis Cassette S, R, and Rz (PVJ4)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 749


Design Notes

The gene cassette consists of the S protein (Holin), R protein (Endolysin), and Rz protein. When paired with an inducible promoter, the cassette is activated by the inducer and begins to produce the proteins which lyse the cell.

The original part name for this construct is PVJ4 (from Harvard's Mekalanos Lab).

Source

This part was designed by Brown University's Team Resistance, consisting of Rima Shah, Katherine Jacobs, and Neil Parikh. It was graciously provided by Vivek Jain and John Mekalanos (Harvard Medical School) who received the original lysis cassette from Ry Young (Texas A&M).

References

Jain, Vivek, Mekalanos, John J. Use of Lambda Phage S and R Gene Products in an Inducible Lysis System for Vibrio cholerae- and Salmonella enterica Serovar Typhimurium-Based DNA Vaccine Delivery Systems Infect. Immun. 2000 68: 986-989

Bonovich, MT, and Ry Young. "Dual Start Motif in Two Lambdoid S Genes Unrelated to Lambda S." Journal of Bacteriology (1991): 2897-2905. PubMed. NCBI. Brown University, Providence. 9 July 2008 <http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=pubmed&list_uids=2019562>.