Part:BBa_K124000:Experience
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K124000
Activation and Repression of CYC1 using scRNAs
Expression control of CYC1 promoter in yeasts.
A histogram shows fluorescence intensities of GFP when it is produced under basal level, activation or repression. The basal level represents cells single-transfected with CYC1-GFP. For the activation, the strain was additionally transfected with dCas9, scCyc1_MS2 and the activation module MCP-VP64. For the repression, the scaffold RNA is scCyc1_PP7 and the transcriptional module is PCP-Mxi1. MS2 targets region c3 of the CYC1 promoter, while PP7 targets region c6. Then, with these basic elements, in principle all gates could be modularly assembled.
User Reviews
UNIQ99b43ac0fcc27ae9-partinfo-00000000-QINU UNIQ99b43ac0fcc27ae9-partinfo-00000001-QINU