Regulatory

Part:BBa_K1231001:Design

Designed by: Brendan Tran   Group: iGEM13_Northwestern   (2013-09-17)

GadA-RBS is a pH sensitive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Genomic PCR from Invitrogen Top10 E. Coli Cells 2010 Wisconsin-Madison iGEM Team inspiration

References

Tramonti, Angela, Michele De Canio, Isabel Delany, Vincenzo Scarlato, and Daniela De Biase. "Mechanisms of Transcription Activation Exerted by GadX and GadW at the GadA and GadBC Gene Promoters of the Glutamate-Based Acid Resistance System in Escherichia Coli." Journal of Bacteriology (2006): n. pag. Web. 27 Sept. 2013.

Waterman, Scott, and P. L. C. Small. "Transcriptional Expression of Escherichia Coli Glutamate-Dependent Acid Resistance Genes GadA and GadBC in an Hns RpoS Mutant†." Journal of Bacteriology (2003): n. pag. Web. 27 Sept. 2013.