Regulatory

Part:BBa_K123002:Design

Designed by: Jason Gardiner   Group: iGEM08_University_of_Alberta   (2008-10-27)


LacIQ ERE TetR


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Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is some data that suggests the ER is toxic to the cell. However in our experiments while we did see a decrease in growth rate we did not find ER to be fatal to the cell.


Source

The Estrogen Responsive Element was taken from the Human Estrogen Responsive Element

References