Translational_Unit

Part:BBa_K1223003:Design

Designed by: Orr Schlesinger   Group: iGEM13_BGU_Israel   (2013-09-07)


Kanamicin ressistance


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

selection gene for that confers the resistance to Kanamycin/Neomycin antibiotics. part is a synthetic construct also used in the registry's pSB3K5 plasmid backbone.

this part was used as a selection gene for the P.A.S.E. 1 and 2 cassettes.

this part contains the promoter, CDS and stop codon.

Source

part was extracted from the pKD4 plasmid and is similar to entry EU496093 on Genbank.

References

Shetty, R.; Endy, D.; Knight, T. Journal of Biological Engineering 2008, 2, 5.