Composite
Part:BBa_K122021:Design
Designed by: David Ouyang Group: iGEM08_Rice_University (2008-10-29)
Cassette3: Constitutive TAL with integration site
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 941
Illegal PstI site found at 1598 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 941
Illegal PstI site found at 1598 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 328
Illegal XhoI site found at 728 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 941
Illegal PstI site found at 1598 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 941
Illegal PstI site found at 1598
Illegal NgoMIV site found at 830
Illegal AgeI site found at 1239 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2027
Design Notes
tPGK for the right side of integration. pADH1 and tADH1 side by side backwards on full construct to minimize unwanted integration.
Source
Rice iGEM