Composite

Part:BBa_K122021:Design

Designed by: David Ouyang   Group: iGEM08_Rice_University   (2008-10-29)


Cassette3: Constitutive TAL with integration site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 941
    Illegal PstI site found at 1598
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 941
    Illegal PstI site found at 1598
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 328
    Illegal XhoI site found at 728
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 941
    Illegal PstI site found at 1598
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 941
    Illegal PstI site found at 1598
    Illegal NgoMIV site found at 830
    Illegal AgeI site found at 1239
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2027


Design Notes

tPGK for the right side of integration. pADH1 and tADH1 side by side backwards on full construct to minimize unwanted integration.


Source

Rice iGEM

References