Regulatory

Part:BBa_K122002:Design

Designed by: David Ouyang   Group: iGEM08_Rice_University   (2008-10-25)

pADH1 (truncated)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 328
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our ADH promoter truncation (containing -700 to 0) allows for constitutive expression throughout the cell life cycle, including during anaerobic fermentation.

Source

Yeast genomic DNA

References

Ruohonen L, Aalto MK, Keränen S. Modifications to the ADH1 promoter of Saccharomyces cerevisiae for efficient production of heterologous proteins. J Biotechnol. 1995 May 1;39(3):193-203. PMID: 7766401 [PubMed - indexed for MEDLINE]