Composite

Part:BBa_K122001:Design

Designed by: David Ouyang   Group: iGEM08_Rice_University   (2008-10-22)

[pGAL1][tetO][ZeoR]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 361
    Illegal BamHI site found at 472
    Illegal XhoI site found at 860
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 757
    Illegal NgoMIV site found at 818
    Illegal AgeI site found at 89
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

glucose, ATC, and tet repressible Zeocin resistance

Source

This construct was generously given to us courtesy of Dmitry Nevozhay from the University of Texas M. D. Anderson Cancer Center.

References