Coding

Part:BBa_K1218018:Design

Designed by: Gordon Wade   Group: iGEM13_Stanford-Brown   (2013-08-29)


Ancestral HisC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Design considerations included making sure to use representative data from Pfam, evaluating any insertions, deletions, or gaps when compared to the starting E coli (K12) sequence, and determining whether these should be kept in the sequence or removed.


Source

This version of HisC is a predicted ancestral sequence. It comes from a prediction made using Pfam seed data (PF00155) as an input for PAML with Lazarus, which is used to predict sequences of a common ancestor.

References