Composite

Part:BBa_K1212009:Design

Designed by: Amy Soon   Group: iGEM13_UC_Davis   (2013-09-16)


pBAD+Riboswitch2+GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 172
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal PstI site found at 172
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 172
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The end of the riboswitch sequence must be directly before the start codon of the coding sequence for GFP. There cannot be a scar.


Source

pBAD and GFP are from the iGEM distribution kit plates and the riboswitch is from the Gallivan Lab's paper A flow cytometry-based screen for synthetic riboswitches. by Lynch et al. in Appl Environ Microbiol.

References

S. A. Lynch and J. P. Gallivan, "A flow cytometry-based screen for synthetic riboswitches," Nucleic Acids Research, vol. 37, pp. 184-192, Jan 2009.