Regulatory

Part:BBa_K1204020:Design

Designed by: Matthew Baron   Group: iGEM13_Duke   (2013-09-16)


TEF1pr-BS3x-16B


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 551
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 551
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 551
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 551
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 172


Design Notes

Had to consider binding rules of iTALs and gRNA/dCas9 complex. Also had to create spacers for multiple binding sites.


Source

Ordered as gene block from IDT

References