Part:BBa_K1202112:Experience

Signal Peptide ecperiments

In this part of our project we tested are our signal peptides is working or not

To secrete GFP protein via signal peptide to extracellular membrane.

In overall project, our aim related with signal peptide is to secrete apoptotic proteins outer membrane. If this construct secrete GFP successfully, that means signal peptide works and TorA can be used in Apoptin or E4orf4 export.

And also we use constitutive promoter in our designs because we need high amount of protein and we want to our bacteria always produce our proteins.

We made santrifuge our signal peptides’ (TorA-GFP ve GFP-HlyA) liquid culture falcons and then get supernatant from falcons. Then we measured values of absorption of GFP in our supernatant in suitable wavelength. Results show that we found high amount of GFP in our supernatants.

Result;

Column scale(y) shows us quantity amount of GFP in the solution.

We get good results from our GFP-HlyA that we found high amount of GFP in our samples more than our Negative(-) Control.

Another good results that our Positive Control which is GFP that added in LB showed maximum amount of GFP in graphics. So we can said that our controls were working too.

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