Coding

Part:BBa_K1202010

Designed by: Furkan Bestepe   Group: iGEM13_ATOMS-Turkiye   (2013-10-04)

LsrR

LsrR is an transcriptional, intercellular peptide that represses Lsr promoter and prevents the transcription phase in E.coli. This repression is inhibited by autoinducer-2 (AI-2) quorum sensing unit phosphorylated by LsrK enzyme.

Lsr operon is one of the existing operons in E.coli for selective production of designated proteins in different conditions. This production chain is regulated strictly by using repressors and activators. In Lsr system, LsrR is the main repressor of the promoter and can only be inhibited by the presence of phosphorylated autoinducer-2 (AI-2) quorum sensing unit. (see diagram) We want to use this part to conduct cancer detecting mechanism in bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 472
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None