Coding

Part:BBa_K120012:Design

Designed by: Lu Yuanye   Group: iGEM08_Peking_University   (2008-10-29)


Gal4(wt)-Triple_lexO


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 716
    Illegal SpeI site found at 2660
    Illegal PstI site found at 1359
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2660
    Illegal PstI site found at 1359
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 228
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 716
    Illegal SpeI site found at 2660
    Illegal PstI site found at 1359
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 716
    Illegal SpeI site found at 2660
    Illegal PstI site found at 1359
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 147
    Illegal BsaI.rc site found at 1823
    Illegal SapI site found at 512


Design Notes

wild type, can active GAL1 promoter


Source

Amplifed from Saccharomyces cerevisiae genomic.


References