Part:BBa_K1199045:Design
LinA-F2A-LinB F2A can cleave LinA and LinB to mediate gamma-HCH
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 532
Illegal XhoI site found at 469 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 694
Illegal NgoMIV site found at 1185
Illegal NgoMIV site found at 1219
Illegal AgeI site found at 619
Illegal AgeI site found at 1285 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
there are restriction enzyme sites BglII and XhoI in the sides of F2A peptide sequence.
Source
LinA and LinB are from Pseudomonas paucimobilis UT26 ,2A is from food and mouth diease virus FMDV
References
1 Ryo Endo, Yoshiyuki Ohtsubo, Masataka Tsuda, and Yuji Nagata, Identification and Characterization of Genes Encoding a Putative ABC-Type Transporter Essential for Utilization of -Hexachlorocyclohexane in Sphingobium japonicum UT26†, JOURNAL OF BACTERIOLOGY, American Society for Microbiology, 9 March 2007 .
2 YUJI NAGATA, TOMOKI NARIYA, RYO OHTOMO, MASAO FUKUDA, KEIJI YANO, AND MASAMICHI TAKAGI ,Cloning and Sequencing of a Dehalogenase Gene Encoding an Enzyme with Hydrolase Activity Involved in the Degradation of y-Hexachlorocyclohexane in Pseudomonaspaucimobilis, JOURNAL OF BACTERIOLOGY, American Society for Microbiology, 9 August 1993.
3 Wei Deng, Dongshan Yang, Bentian Zhao, Zhen Ouyang, Jun Song, Nana Fan, Zhaoming Liu, Yu Zhao, Qinghong Wu,Bayaer Nashun, Jiangjing Tang, Zhenfang Wu, Weiwang Gu, Liangxue Lai, Use of the 2A Peptide for Generation of Multi-Transgenic Pigs through a Single Round of Nuclear Transfer. Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou. Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China, Institute of Comparative Medicine and Center of Laboratory Animals, Southern Medical University, Guangzhou, China. College of Animal Science, South China Agricultural University, Guangzhou, China.
4 1.Nagata Y, Natsui S, Endo R, Ohtsubo Y, Ichikawa N, Ankai A, Oguchi A, Fukui S, Fujita N, Tsuda M.Genomic organization and genomic structural rearrangements of Sphingobium japonicum UT26, an archetypal γ-hexachlorocyclohexane-degrading bacterium.2011 Dec 10;49(6-7):499-508. doi: 10.1016/j.enzmictec.2011.10.005. Epub 2011 Nov 7.