Coding

Part:BBa_K1199045

Designed by: Tian Wang   Group: iGEM13_UESTC_Life   (2013-09-17)

LinA-F2A-LinB F2A can cleave LinA and LinB to mediate gamma-HCH

F2A peptide sequence is in foot and mouth diease virus gene.it link LinA and LinB gene and in the end of LinA gene don't have stop coden. When it is expressd in E.coli, LinA and LinB are cleaved by F2A peptide. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 532
    Illegal XhoI site found at 469
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 694
    Illegal NgoMIV site found at 1185
    Illegal NgoMIV site found at 1219
    Illegal AgeI site found at 619
    Illegal AgeI site found at 1285
  • 1000
    COMPATIBLE WITH RFC[1000]


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