DNA

Part:BBa_K1195010:Design

Designed by: Lindsey Perry, Xiuqi Li   Group: iGEM13_BYU_Provo   (2013-10-25)


T7 Bacteriophage Minor Capsid Protein (Small Mutant 1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 292
    Illegal BsaI.rc site found at 497


Design Notes

N/A


Source

We boiled T7 bacteriophage for 5 minutes to expose the genome and then ran PCR to obtain the minor capsid protein gene.

References