Tag

Part:BBa_K1188002:Design

Designed by: Jean-Francois Lalonde   Group: iGEM13_CU-Boulder   (2013-08-23)


RTX - Calcium Binding Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 223
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We ordered PCR primers to isolate RTX from its original construct. Due to the repetitive nature of this construct, we found that there were multiple binding sites for the forward primer which resulted in two products. This is the shorter PCR product, with only 8 repeats, whereas the original construct had 17.


Source

J Biol Chem. 2011 May 13;286(19):16997-7004

http://www.ncbi.nlm.nih.gov/pubmed/21454565

References