Signalling

Part:BBa_K1180003:Design

Designed by: Chen Xi, Niu Yuchen,Dai Yimei   Group: iGEM13_NJU_China   (2013-09-24)


Fusion protein of lamp 2b and pre-s1 for liver targeting of the exosome


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1390
    Illegal SpeI site found at 703
    Illegal PstI site found at 1087
    Illegal PstI site found at 1473
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 703
    Illegal PstI site found at 1087
    Illegal PstI site found at 1473
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1872
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1390
    Illegal SpeI site found at 703
    Illegal PstI site found at 1087
    Illegal PstI site found at 1473
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1390
    Illegal SpeI site found at 703
    Illegal PstI site found at 1087
    Illegal PstI site found at 1473
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 334
    Illegal BsaI site found at 1567


Design Notes

We tried to make the pre-S1 sequence as short as possible in order to avoid any immune response which might be induced by the fusion protein.


Source

The sequence of our part come from the protein lamp 2b and pre-S1 from HBV envelope protein.

References