Signalling
Part:BBa_K1180002:Design
Designed by: Gong Fei ,Wang Wei, Wei Xiuqing Group: iGEM13_NJU_China (2013-09-24)
fusion protein of lamp 2b and RVG for brain targeting of the exosome
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1135
Illegal SpeI site found at 448
Illegal PstI site found at 832
Illegal PstI site found at 1218 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 448
Illegal PstI site found at 832
Illegal PstI site found at 1218 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1617
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1135
Illegal SpeI site found at 448
Illegal PstI site found at 832
Illegal PstI site found at 1218 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1135
Illegal SpeI site found at 448
Illegal PstI site found at 832
Illegal PstI site found at 1218 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1312
Illegal BsaI.rc site found at 150
Design Notes
We need to make the RVG peptide as short as possible to avoid the immune response which might be induced by the fusion protein.
Source
The sequence is from the protein lamp 2b and RVG peptide.