Generator

Part:BBa_K1179073:Design

Designed by: Nelson Hall   Group: iGEM13_MIT   (2013-09-18)


TRE-tight_eYFP-4x451


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1229
    Illegal XbaI site found at 30
    Illegal SpeI site found at 1205
    Illegal PstI site found at 590
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1229
    Illegal SpeI site found at 1205
    Illegal PstI site found at 590
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1229
    Illegal BamHI site found at 353
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1229
    Illegal XbaI site found at 30
    Illegal SpeI site found at 1205
    Illegal PstI site found at 590
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1229
    Illegal XbaI site found at 30
    Illegal SpeI site found at 1205
    Illegal PstI site found at 590
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Not applicable

Source

Constructed by performing a gateway reaction with a TRE-tight promoter and the eYFP-4x451ts entry vectors. See components for more information.

References