Generator

Part:BBa_K1179027:Design

Designed by: Lauren St. Hilaire   Group: iGEM13_MIT   (2013-09-15)


CAG_tagBFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 2581
    Illegal XbaI site found at 1634
    Illegal XbaI site found at 2624
    Illegal PstI site found at 2586
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 2581
    Illegal PstI site found at 2586
    Illegal NotI site found at 2475
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 2581
    Illegal BamHI site found at 2612
    Illegal XhoI site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 2581
    Illegal XbaI site found at 1634
    Illegal XbaI site found at 2624
    Illegal PstI site found at 2586
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1730
    Illegal EcoRI site found at 2581
    Illegal XbaI site found at 1634
    Illegal XbaI site found at 2624
    Illegal PstI site found at 2586
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 1479
    Illegal AgeI site found at 1769
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2403


Design Notes

Not applicable

Source

Constructed by performing a Gateway reaction with a CAG promoter and the tagBFP entry vector. TagBFP is a monomeric blue fluorescent protein generated by site-specific and random mutagenesis of TagRFP

References