Generator

Part:BBa_K1179025:Design

Designed by: Lauren St. Hilaire   Group: iGEM13_MIT   (2013-09-15)


CAG_L7Ae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1730
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1730
    Illegal XhoI site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 1479
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Not applicable

Source

Constructed by performing a Gateway reaction with the CAG promoter and L7Ae entry vector.

References