Part:BBa_K1179015:Design

A U6_gRNA(Cr9) plasmid which produces Cr9 targeting guide RNA's for Cas9

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Make sure the U6 terminator is present and that the target sequence being driven by the U6 promoter starts with a G and is 20 base pairs long.

Source

Sincere thanks to Samira Kiani for contributing this entry vector to the 2013 MIT iGEM team.