Coding

Part:BBa_K1179006:Design

Designed by: Nelson Hall   Group: iGEM13_MIT   (2013-09-15)


A backbone for ligation of the Acyl-TyA domain to a protein of your choice


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Keep in mind that the Acyl-TyA sequence exports the protein into exosomes so protein extraction and other quantification protocols are needed to test successful experimental function. It also has a TEV cleaving site between the BsaI site and the TyA sequence.

Source

Derived from a yeast retrotransposon virus-like particle. Synthesized with the addition of a Tev cleaving site after the TyA sequence.

References