Device

Part:BBa_K1172588:Design

Designed by: Thorben Meyer   Group: iGEM13_Bielefeld-Germany   (2013-10-25)

Combination of oprF and rib-synth coding sequence under control of T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 802
    Illegal XhoI site found at 2456
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1194
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 715


Design Notes

In order to combine the oprF BioBrick under control of the T7 promoter BBa_K1172502 with the rib-gene-cluster BBa_K1172303 we used the [http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Standard_BioBrick_Assembly Standard BioBrick Assembly Suffix Insertion].



Source

References