Intermediate
Part:BBa_K117007:Design
Designed by: Nguyen Xuan Hung Group: iGEM08_NTU-Singapore (2008-10-08)
colE7ImmE7-double terminator BBa_B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Colicin E7 is known to degrade chromosomal and plasmid DNA. The rate at which the degradation occurs was found to be directly proportional to E7 concentration.
Source
Part BBa_K117001 was constructed by NTU iGEM 2008 team. Part BBa_B0015 was obtained from the Registry
References
Folia Microbiol. 35, 348-352 (1990). Colicins E7 and E8 Degrade DNA. in Sensitive Bacteria. J. MARDA', J. MARDA Jr. h and Z. VRBICKA
Structure. Volume 7, Issue 1, 15 January 1999, Pages 91-102 T.P. Ko, C. C. Liao, W. Y. Ku, K. F. Chak and H. S. Yuan, The crystal structure of the DNase domain of colicin E7 in complex with its inhibitor Im7 protein