Regulatory

Part:BBa_K1170003:Design

Designed by: Rohit Satija   Group: iGEM13_IIT_Delhi   (2013-09-04)

p-atp2 in F0F1 ATPase operon from C. glutamicum ATCC 13032


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 625
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 625
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 625
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 625
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 625
    Illegal AgeI site found at 30
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The exact minimal promoter region of P-atp2 is unavailable in literature.


Source

PCR amplified from the genome of C. glutamicum ATCC 13032.

References

Mo´nicaBarriuso-Iglesias, Carlos Barreiro, Fabio Flechosoand Juan F. Martı´n, Transcriptional analysis of the F0F1 ATPase operonof Corynebacterium glutamicum ATCC 13032reveals strong induction by alkaline pH. Microbiology January 2006 152:1 11-21