Reporter

Part:BBa_K1170002

Designed by: Rohit Satija   Group: iGEM13_IIT_Delhi   (2013-09-17)


lacZ (gi: J01636.1, Encoding Beta-Galactosidase Enzyme), complete cds

lacZ gene encodes for β-Galactosidase enzyme which hydrolyses the β-(1,4) bond between a glucose unit(α or β form) and a galactose unit(only β form). The intracellular enzyme can be used to react with X-Gal as substrate and produce a blue coloured dye to act as a visible reporter of lacZ gene expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None