Coding

Part:BBa_K116402:Design

Designed by: Chih-Hsien Yang   Group: iGEM08_NYMU-Taipei   (2008-10-29)


polyphosphate kinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 317
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1671
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

WT ppk gene in E.coli has a internal biobrick standard cutting site "GAA TTC" (EcoRI site). Thus we design an set of internal primers to replace the EcoRI site "GAA TTC" as "GAG TTT." This ppk mutant can be applied in biobrick standard assembly.


Source

E. coli K12 genomic DNA

References