Coding
Part:BBa_K116402:Design
Designed by: Chih-Hsien Yang Group: iGEM08_NYMU-Taipei (2008-10-29)
polyphosphate kinase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 317
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1671
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
WT ppk gene in E.coli has a internal biobrick standard cutting site "GAA TTC" (EcoRI site). Thus we design an set of internal primers to replace the EcoRI site "GAA TTC" as "GAG TTT." This ppk mutant can be applied in biobrick standard assembly.
Source
E. coli K12 genomic DNA