Plasmid

Part:BBa_K1160000:Design

Designed by: Huang Xingxu   Group: iGEM13_NJU_NJUT_China   (2013-09-13)


coding sequence of Cas9 from CRISPR system type II


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 249
    Illegal PstI site found at 3115
    Illegal PstI site found at 4561
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 895
    Illegal SpeI site found at 249
    Illegal PstI site found at 3115
    Illegal PstI site found at 4561
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
    Illegal BglII site found at 1154
    Illegal BglII site found at 4479
    Illegal BamHI site found at 2243
    Illegal XhoI site found at 2041
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 249
    Illegal PstI site found at 3115
    Illegal PstI site found at 4561
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 249
    Illegal PstI site found at 3115
    Illegal PstI site found at 4561
    Illegal NgoMIV site found at 3643
    Illegal NgoMIV site found at 5515
    Illegal NgoMIV site found at 6775
    Illegal AgeI site found at 5068
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5050
    Illegal BsaI.rc site found at 882
    Illegal BsaI.rc site found at 8303
    Illegal SapI site found at 2556
    Illegal SapI site found at 7220
    Illegal SapI.rc site found at 2062
    Illegal SapI.rc site found at 2800
    Illegal SapI.rc site found at 3777


Design Notes

Source

Streptococcus pyogenes

References