Part:BBa_K1159301

Super Yellow Fluorescent Protein 2 (SYFP2) in RFC[25]

Improved part of BBa_K864100 which originally cannot be fused to other protein domains. This improved version is flanked by RFC[25] pre- and suffix for further protein fusions.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Protein data table for BioBrick BBa_ automatically created by the BioBrick-AutoAnnotator version 1.0

Nucleotide sequence in RFC 25, so ATGGCCGGC and ACCGGT were added (in italics) to the 5' and 3' ends: (underlined part encodes the protein)
ATGGCCGGCGTTAGCAAG ... CTGTATAAAACCGGT
ORF from nucleotide position -8 to 720 (excluding stop-codon)

Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)

1
101
201

MAGVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERT
IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPD
NHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKTG*

Sequence features: (with their position in the amino acid sequence, see the list of supported features)

None of the supported features appeared in the sequence

Amino acid composition:

Ala (A)	10 (4.1%)
Arg (R)	6 (2.5%)
Asn (N)	13 (5.3%)
Asp (D)	18 (7.4%)

Cys (C)	2 (0.8%)
Gln (Q)	8 (3.3%)
Glu (E)	16 (6.6%)
Gly (G)	26 (10.7%)

His (H)	9 (3.7%)
Ile (I)	12 (4.9%)
Leu (L)	22 (9.1%)
Lys (K)	21 (8.6%)

Met (M)	5 (2.1%)
Phe (F)	11 (4.5%)
Pro (P)	10 (4.1%)
Ser (S)	8 (3.3%)

Thr (T)	16 (6.6%)
Trp (W)	1 (0.4%)
Tyr (Y)	12 (4.9%)
Val (V)	17 (7.0%)

Amino acid counting

	Total number:	243
	Positively charged (Arg+Lys):	27 (11.1%)
	Negatively charged (Asp+Glu):	34 (14.0%)
	Aromatic (Phe+His+Try+Tyr):	33 (13.6%)

Biochemical parameters

	Atomic composition:	C₁₂₁₈H₁₈₈₂N₃₂₂O₃₆₉S₇
	Molecular mass [Da]:	27164.7
	Theoretical pI:	5.71
	Extinction coefficient at 280 nm [M^-1 cm^-1]:	23380 / 23505 (all Cys red/ox)

Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges

Codon usage

	Organism:	E. coli	B. subtilis	S. cerevisiae	A. thaliana	P. patens	Mammals
	Codon quality (CAI):	good (0.80)	good (0.76)	good (0.63)	good (0.70)	excellent (0.81)	good (0.72)

Alignments (obtained from PredictProtein.org)

	SwissProt:	P42212 (95% identity on 238 AAs), Q9U6Y3 (26% identity on 217 AAs)
	TrEML:	B6UPG7 (96% identity on 238 AAs), B7UCZ6 (96% identity on 238 AAs)
	PDB:	1myw (97% identity on 228 AAs), 1qxt (96% identity on 226 AAs)

Predictions (obtained from PredictProtein.org)

Subcellular Localization (reliability in brackets)

	Archaea:	secreted (100%)
	Bacteria:	cytosol (50%)
	Eukarya:	cytosol (36%)

Gene Ontology (reliability in brackets)

	Molecular Function Ontology:	-
	Biological Process Ontology:	GO:0018298 (44%), GO:0008218 (31%)

Predicted features:

	Disulfid bridges:	-
	Transmembrane helices:	-

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