Part:BBa_K1159004

Human protein phosphatase 1 (PP1) in RFC[25]

Human protein phosphatase 1 binds the toxic oligopeptide produces by gree-blue algae. This part is flanked by RFC[25] pre- and suffix.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 537
  Illegal BamHI site found at 1
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 924
  

Protein data table for BioBrick BBa_ automatically created by the BioBrick-AutoAnnotator version 1.0
Nucleotide sequence in RFC 25 N-Part using the stop codon in the suffix, so ACCGGT was added (in italics) to the 3' end: (underlined part encodes the protein)  GGATCCGCGGATTTAGATAAACTCAACATCGACAGCATTATCCAACGGCTGCTGGAAGTGAGAGGGTCCAAGCCTGGTAAGAATGTCCAGC ... TTCAGGAGAACCGGT  ORF from nucleotide position 83 to 106 (excluding stop-codon)
Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below) 1  MSSFRRTG*
Sequence features: (with their position in the amino acid sequence, see the list of supported features) None of the supported features appeared in the sequence
Amino acid composition: Ala (A) 0 (0.0%) Arg (R) 2 (25.0%) Asn (N) 0 (0.0%) Asp (D) 0 (0.0%) Cys (C) 0 (0.0%) Gln (Q) 0 (0.0%) Glu (E) 0 (0.0%) Gly (G) 1 (12.5%) His (H) 0 (0.0%) Ile (I) 0 (0.0%) Leu (L) 0 (0.0%) Lys (K) 0 (0.0%) Met (M) 1 (12.5%) Phe (F) 1 (12.5%) Pro (P) 0 (0.0%) Ser (S) 2 (25.0%) Thr (T) 1 (12.5%) Trp (W) 0 (0.0%) Tyr (Y) 0 (0.0%) Val (V) 0 (0.0%)
Amino acid counting Total number: 8 Positively charged (Arg+Lys): 2 (25.0%) Negatively charged (Asp+Glu): 0 (0.0%) Aromatic (Phe+His+Try+Tyr): 1 (12.5%)	Biochemical parameters Atomic composition: C38H64N14O12S1 Molecular mass [Da]: 941.1 Theoretical pI: 12.00 Extinction coefficient at 280 nm [M-1 cm-1]: 0 / 0 (all Cys red/ox)
Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges
Codon usage Organism: E. coli B. subtilis S. cerevisiae A. thaliana P. patens Mammals Codon quality (CAI): good (0.63) acceptable (0.59) good (0.65) good (0.68) excellent (0.88) excellent (0.91)
Alignments (obtained from PredictProtein.org)    There were no alignments for this protein in the data base. The BLAST search was initialized and should be ready in a few hours.
Predictions (obtained from PredictProtein.org)
There were no predictions for this protein in the data base. The prediction was initialized and should be ready in a few hours.
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