Composite

Part:BBa_K1157020:Design

Designed by: Lin Ang Chieh   Group: iGEM13_NTU-Taida   (2013-09-15)


Pc-RhlR-pRhl-RhlR-GFPmut3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 304
    Illegal BamHI site found at 1260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 779
    Illegal BsaI site found at 1735
    Illegal BsaI.rc site found at 2422


Design Notes

The sequences are confirmed and there is no restriction enzyme sites in the sequence.


Source

It is built by iGEM official biobrick

References