Part:BBa_K1157019:Experience

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Applications of BBa_K1157019

We(2013 igem NTU_Taida) tested the function of the combination of BBa_K1157017 an BBa_K1157019. We used clinical bacterial strains to test this biosensor's activity. These as the clinical species we used. (1) Acicetobacter baumannii ATCC baa-747 (2) Pseudomonas aeruginosa PAO 1 (3) Pseudomonas aeruginosa 1009373 (4) Pseudomonas aeruginosa 2000671 (5) Pseudomonas aeruginosa 0000765-1 (6) Pseudomonas aeruginosa 0000963 (7) Pseudomonas aeruginosa 0001596 (8) Pseudomonas aeruginosa 0001819 (9) Acicetobacter baumannii 8070903 (10) Acicetobacter baumannii 0008115 (11) Escherichia coli 0000424 (12) Escherichia coli 0000703 We transformed this biobrick in E. Coli DH5α, and along with the clinical species we culture it overnight. Then we dilute our biosensor until OD=0.1 and centrifuge the clinical species to use its supernatant as our target. We used ELISA plate reader to read the fluorescence after 4 hours of adding the supernatant. It is obvious that the some strains have stronger fluorescence while others have almost no reaction at all.

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