Composite
Part:BBa_K1157015:Experience
Designed by: Lin Ang Chieh Group: iGEM13_NTU-Taida (2013-09-15)
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1157015
We tested this circuit by transforming it in E. Coli DH5 alfa. We cultured the E. Coli in LB medium overnight, dilute until OD value=0.1, then added into ELISA plate reader for testing.(See testing protocol in NTU_Taida igem WIKI for details.) We tested different concentration of C-4 AHL to see whether the constitutive production of fluorescent protein will be inhibited through the CI-pCI circuit. The concentration unit is M. This is our functional results of testing BBa_K1157015 for 4 hours.
User Reviews
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