Composite

Part:BBa_K1157015

Designed by: Lin Ang Chieh   Group: iGEM13_NTU-Taida   (2013-09-15)

pConst-Rhl-pRhl-RhlR-CI-pCI-mCherry

This biosensor is constructed to sense C-4 AHL molecules through a different method. This time when AHL molecules are sensed, it activates Rhl promoter, which initiates the production of CI. Therefore, CI inhibits pCI and the expression of reporter mCherry is stopped. When compared to control group, the results will be expected to express lower fluorescence level when there is existence of AHL molecules.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 304
    Illegal BamHI site found at 1260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 779
    Illegal BsaI site found at 1735


[edit]
Categories
Parameters
None