Composite
pCI-mCherr

Part:BBa_K1157013

Designed by: Lin Ang Chieh   Group: iGEM13_NTU-Taida   (2013-09-15)

pCI-mCherry-pConst-RhlR-pRhl-RhlR-CI

The construct of this circuit is supported by igem team NTU_Taiwan. This biosensor is constructed to sense C-4 AHL molecules through a method different from BBa_K1157012. This time when AHL molecules are sensed, it activates Rhl promoter, which initiates the production of CI. Therefore, CI inhibits pCI and the expression of reporter mCherry is stopped. When compared to control group, the results will be expected to express lower fluorescence level when there is existence of AHL molecules.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 944
    Illegal NheI site found at 967
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1241
    Illegal BamHI site found at 2197
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1716
    Illegal BsaI site found at 2672


[edit]
Categories
//function/cellsignalling
Parameters
None